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HBMEC細(xì)胞|美國(guó)Sciencell|人腦微血管內(nèi)皮細(xì)胞 全國(guó)統(tǒng)一價(jià)格,全國(guó)各地均可發(fā)貨,遠(yuǎn)到香港、澳門、福建、新疆、內(nèi)蒙古等地。
產(chǎn)品名稱:HBMEC細(xì)胞
中文名稱:人腦微血管內(nèi)皮細(xì)胞
來源:美國(guó)Sciencell
生長(zhǎng)狀態(tài):貼壁生長(zhǎng)|懸浮生長(zhǎng)|半貼壁半懸浮生長(zhǎng)
細(xì)胞形態(tài):上皮樣|成纖維樣|圓形|不規(guī)則
培養(yǎng)條件:DMEM|1640|MEM|IMDM+10% FBS
細(xì)胞規(guī)格:5 x 10^5 cells/vial
特征特性:
人腦微血管內(nèi)皮細(xì)胞是血腦屏障的主要組成成分,能夠限制可溶性物質(zhì)和細(xì)胞等從血液進(jìn)入大腦。大腦微血
管內(nèi)皮細(xì)胞與外周內(nèi)皮細(xì)胞相比具有一些相同特性。如:1)腦微血管內(nèi)皮細(xì)胞存在許多細(xì)胞間緊密連接,
產(chǎn)生很高的跨內(nèi)皮阻抗,延遲細(xì)胞旁的通量;2)腦微血管內(nèi)皮細(xì)胞缺乏內(nèi)皮細(xì)胞的窗孔結(jié)構(gòu),其液相物質(zhì)
胞飲水平較低;3)腦微血管內(nèi)皮細(xì)胞具有不對(duì)稱定位酶和載體介導(dǎo)轉(zhuǎn)運(yùn)系統(tǒng),從而產(chǎn)生 “兩極分化”的表現(xiàn)型。
與外周內(nèi)皮細(xì)胞相同,大腦微血管內(nèi)皮細(xì)胞表面表達(dá)細(xì)胞粘附分子,調(diào)控白細(xì)胞進(jìn)入大腦。由于微血管內(nèi)皮細(xì)
胞的器官特異性,內(nèi)皮細(xì)胞通常取源于疾病研究的相關(guān)組織。
HBMEC細(xì)胞提取于人腦組織,提取純化之后立即凍存。每管含有細(xì)胞數(shù) >5×105 cells/ml,
此細(xì)胞通過vWF/Factor VIII 和CD31 (P-CAM)免疫熒光染色驗(yàn)證,經(jīng)測(cè)試不含有HIV-1、HBV、HCV、支原體、
細(xì)菌、酵母和真菌。
庫存狀態(tài):現(xiàn)貨
運(yùn)輸方式:運(yùn)輸方式分為新鮮運(yùn)輸和凍存管運(yùn)輸兩種運(yùn)輸方式
貨期:新鮮運(yùn)輸需要1-2周,凍存管運(yùn)輸?shù)男枰?-3天
慧穎細(xì)胞庫提供的所有細(xì)胞僅供科研使用
原代細(xì)胞的培養(yǎng)是指直接從機(jī)體取下細(xì)胞、組織和器官后立即進(jìn)行培 養(yǎng);因此,較為嚴(yán)格地說是指成功傳代之前的培養(yǎng),此時(shí)的細(xì)胞保持原有細(xì)胞的基本性質(zhì),如果是正常細(xì)胞,仍然保留二倍體數(shù)。不同的原代細(xì)胞傳代次數(shù)不同,具體需咨詢客服人員或者自行查閱 相關(guān)資料。
細(xì)胞株是通過選擇法或克隆形成法從原代培養(yǎng)物或細(xì)胞系中獲得的具有特殊性質(zhì)或標(biāo)志的培 養(yǎng)細(xì)胞。從培養(yǎng)代數(shù)來講,理論上可培養(yǎng)到40-50代。
客戶應(yīng)具有一定細(xì)胞培養(yǎng)知識(shí)與經(jīng)驗(yàn),并具備基本培養(yǎng)條件(細(xì)胞培養(yǎng)實(shí)驗(yàn)室,無菌操作臺(tái)、CO2培養(yǎng)箱,可拍照倒置顯微鏡等),并按說明書要求準(zhǔn)備好相應(yīng)的無菌的培養(yǎng)基、血清、雙抗、細(xì)胞培養(yǎng)瓶、培養(yǎng)板等。如果因客戶缺少基本培養(yǎng)經(jīng)驗(yàn)和培養(yǎng)條件而導(dǎo)致細(xì)胞死亡或污染,不在我司售后責(zé)任之內(nèi)。
相關(guān)HBMEC細(xì)胞|美國(guó)Sciencell|人腦微血管內(nèi)皮細(xì)胞熱賣產(chǎn)品:
骨、關(guān)節(jié)、軟組織和皮膚系統(tǒng)Bones, joints,soft tissue and skin system
BJ細(xì)胞,人原代成纖維細(xì)胞 5 × 105 1-2weeks
HSF細(xì)胞, 人皮膚成纖維細(xì)胞 5 × 105 1-2weeks
FLS細(xì)胞, 成纖維樣滑膜細(xì)胞 5 × 105 1-2weeks
A-431細(xì)胞, 人皮膚鱗癌細(xì)胞 5 × 105 1-2weeks
SK-MEL-1 細(xì)胞,人皮膚黑色素瘤細(xì)胞 5 × 105 1-2weeks
A875細(xì)胞, 人黑色素瘤細(xì)胞 5 × 105 1-2weeks
A-375細(xì)胞, 人惡性黑色素瘤細(xì)胞 5 × 105 1-2weeks
HaCaT細(xì)胞, 人永生化表皮細(xì)胞 5 × 105 1-2weeks
hFOB1.19 細(xì)胞,人永生化成骨細(xì)胞 5 × 105 1-2weeks
hFOB 1.19細(xì)胞, SV40轉(zhuǎn)染人成骨細(xì)胞 5 × 105 1-2weeks
HT-1080細(xì)胞,人纖維肉瘤細(xì)胞 5 × 105 1-2weeks
MG-63細(xì)胞,人成骨肉瘤細(xì)胞 5 × 105 1-2weeks
Saos-2細(xì)胞, 人成骨肉瘤細(xì)胞 5 × 105 1-2weeks
TE671 subline No.2 人橫紋肌肉瘤細(xì)胞 5 × 105 1-2weeks
U266細(xì)胞, 人骨髓瘤細(xì)胞 5 × 105 1-2weeks
HOS細(xì)胞, 人骨肉瘤細(xì)胞 5 × 105 1-2weeks
U-2 OS細(xì)胞,人骨肉瘤細(xì)胞 5 × 105 1-2weeks
h-BMSC細(xì)胞,人骨髓間質(zhì)細(xì)胞 5 × 105 1-2weeks
呼吸系統(tǒng)respiratory system
16HBE 人支氣管上皮樣細(xì)胞 5 × 105 1-2weeks
95-D 人高轉(zhuǎn)移肺癌細(xì)胞 5 × 105 1-2weeks
H322 肺癌細(xì)胞 5 × 105 1-2weeks
A549 人肺癌細(xì)胞 5 × 105 1-2weeks
H1299(中科:NCI-H1299) 人肺癌細(xì)胞 5 × 105 1-2weeks
PC9 人肺癌細(xì)胞 5 × 105 1-2weeks
PC14 人肺癌細(xì)胞 5 × 105 1-2weeks
SPCA-1 人肺腺癌細(xì)胞 5 × 105 1-2weeks
Calu-3 人肺腺癌細(xì)胞 5 × 105 1-2weeks
LTEP-a-2 人肺腺癌細(xì)胞 5 × 105 1-2weeks
MRC-5 人胚肺成纖維細(xì)胞 5 × 105 1-2weeks
WI-38 人胚胎肺成纖維細(xì)胞 5 × 105 1-2weeks
A549(成瘤) 人非小細(xì)胞肺癌細(xì)胞 5 × 105 1-2weeks
NCI-H358 人非小細(xì)胞肺癌細(xì)胞 5 × 105 1-2weeks
NCI-H1650 人非小細(xì)胞肺癌細(xì)胞 5 × 105 1-2weeks
NCI-H1975 人非小細(xì)胞肺腺癌細(xì)胞 5 × 105 1-2weeks
NCI-N838 人非小細(xì)胞肺癌細(xì)胞 5 × 105 1-2weeks
SK-MES-1 人肺鱗癌細(xì)胞 5 × 105 1-2weeks
NCI-H226 人肺鱗癌細(xì)胞 5 × 105 1-2weeks
NCI-H520 人肺鱗癌細(xì)胞 5 × 105 1-2weeks
NCI-H460 人大細(xì)胞肺癌細(xì)胞 5 × 105 1-2weeks
NCI-H661 人大細(xì)胞肺癌細(xì)胞 5 × 105 1-2weeks
泌尿系統(tǒng)urinary system
293 人胚腎細(xì)胞 5 × 105 1-2weeks
293A 人胚腎細(xì)胞 5 × 105 1-2weeks
293F 人胚腎細(xì)胞 5 × 105 1-2weeks
HEK293 人胚腎細(xì)胞 5 × 105 1-2weeks
293XL-hTLR7 人胚腎細(xì)胞 5 × 105 1-2weeks
293XL-hTLR9 人胚腎細(xì)胞 5 × 105 1-2weeks
293-mTLR5 人胚腎細(xì)胞 5 × 105 1-2weeks
293 xhTLR7 人胚腎細(xì)胞 5 × 105 1-2weeks
293 xhTLR9 人胚腎細(xì)胞 5 × 105 1-2weeks
293H 人胚腎細(xì)胞 5 × 105 1-2weeks
293FT 人胚腎細(xì)胞-用于慢病毒包裝 5 × 105 1-2weeks
293TN 人胚腎細(xì)胞--用于慢病毒包裝 5 × 105 1-2weeks
293T/17 人胚腎細(xì)胞-用于慢病毒包裝 5 × 105 1-2weeks
293LVT 人胚腎細(xì)胞--用于慢病毒包裝 5 × 105 1-2weeks
293T 人胚腎細(xì)胞(SV40T基因修飾) 5 × 105 1-2weeks
LNCaP clone FGC 人前列腺癌細(xì)胞 5 × 105 1-2weeks
DU 145 人前列腺癌細(xì)胞 5 × 105 1-2weeks
786-O 人腎透明細(xì)胞癌細(xì)胞 5 × 105 1-2weeks
Caki-1 人腎透明細(xì)胞癌皮膚轉(zhuǎn)移細(xì)胞 5 × 105 1-2weeks
G401 人腎癌Wilms細(xì)胞 5 × 105 1-2weeks
HK-2 人腎皮質(zhì)近曲小管上皮細(xì)胞 5 × 105 1-2weeks
ADPC 人雄激素依靠性前列腺癌細(xì)胞 5 × 105 1-2weeks
RWPE-1 人正常前列腺上皮細(xì)胞 5 × 105 1-2weeks
T24 人膀胱癌細(xì)胞 5 × 105 1-2weeks
EJ 人膀胱癌細(xì)胞 5 × 105 1-2weeks
5637 人膀胱癌細(xì)胞 5 × 105 1-2weeks
ScaBER 人膀胱鱗癌細(xì)胞 5 × 105 1-2weeks
內(nèi)分泌系統(tǒng)endocrine system
BT-474 人乳腺導(dǎo)管癌細(xì)胞 5 × 105 1-2weeks
435(中科:MDA-MB-435S ) 人乳腺導(dǎo)管癌 5 × 105 1-2weeks
HS-578T 人乳腺癌細(xì)胞 5 × 105 1-2weeks
MCF-7 人乳腺癌細(xì)胞 5 × 105 1-2weeks
MDA-MB-231 人乳腺癌細(xì)胞 5 × 105 1-2weeks
T47D 人乳腺癌細(xì)胞 5 × 105 1-2weeks
LLC 人乳腺癌細(xì)胞 5 × 105 1-2weeks
MCF 7 人乳腺癌細(xì)胞 5 × 105 1-2weeks
MDA-MB-231 人乳腺癌細(xì)胞 5 × 105 1-2weeks
SKBR3 人乳腺腺癌細(xì)胞 5 × 105 1-2weeks
MCF 10A 人正常乳腺上皮細(xì)胞 5 × 105 1-2weeks
SW579 人甲狀腺鱗癌細(xì)胞 5 × 105 1-2weeks
TT 人髓狀甲狀腺腫瘤細(xì)胞 5 × 105 1-2weeks
腦和神經(jīng)系統(tǒng)The brain and nervous system
A172 人膠質(zhì)母細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
BE(2)-M17 人神經(jīng)母細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
IMR-32 人神經(jīng)母細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
SH-SY5Y 人神經(jīng)母細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
SK-N-SH 人神經(jīng)母細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
SHG-44 人膠質(zhì)瘤細(xì)胞 5 × 105 1-2weeks
HS 683 人腦膠質(zhì)瘤細(xì)胞 5 × 105 1-2weeks
U-373MG 人腦神經(jīng)膠質(zhì)瘤細(xì)胞 5 × 105 1-2weeks
U251 人神經(jīng)膠質(zhì)細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
U-87 MG 人腦星形膠質(zhì)母細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
生殖系統(tǒng)reproductive system
C-33A 人宮頸癌細(xì)胞 5 × 105 1-2weeks
Ca Ski 人宮頸癌細(xì)胞 5 × 105 1-2weeks
Hela229 人宮頸癌細(xì)胞 5 × 105 1-2weeks
HeLa 人宮頸癌細(xì)胞 5 × 105 1-2weeks
人胚胎成纖維細(xì)胞 5 × 105 1-2weeks
HEC-1-B 人子宮內(nèi)膜腺癌細(xì)胞 5 × 105 1-2weeks
Ishikawa 人子宮內(nèi)膜癌細(xì)胞 5 × 105 1-2weeks
SKOV3 人卵巢癌細(xì)胞 5 × 105 1-2weeks
HO-8910 人卵巢癌細(xì)胞 5 × 105 1-2weeks
SK-OV-3 人卵巢腺癌細(xì)胞 5 × 105 1-2weeks
OVCAR-3 人卵巢腺癌細(xì)胞 5 × 105 1-2weeks
JEG3 人絨毛膜癌細(xì)胞 5 × 105 1-2weeks
Ms751 人子宮頸表皮癌細(xì)胞 5 × 105 1-2weeks
Siha 人子宮頸鱗癌細(xì)胞 5 × 105 1-2weeks
KLE 人子宮內(nèi)膜腺癌細(xì)胞 5 × 105 1-2weeks
消化系統(tǒng)digestive system
PANC-1細(xì)胞,人胰腺癌細(xì)胞 5 × 105 1-2weeks
SW1990細(xì)胞, 人胰腺癌細(xì)胞 5 × 105 1-2weeks
BxPC-3細(xì)胞,人胰腺腺癌細(xì)胞 5 × 105 1-2weeks
chang liver細(xì)胞, 人張氏肝細(xì)胞 5 × 105 1-2weeks
HIC-2細(xì)胞, 人小腸癌細(xì)胞 5 × 105 1-2weeks
HCT-8細(xì)胞,人回盲腸癌細(xì)胞 5 × 105 1-2weeks
SW620[SW-620] 細(xì)胞,人結(jié)腸癌細(xì)胞 5 × 105 1-2weeks
HCT-116細(xì)胞,人結(jié)腸癌細(xì)胞 5 × 105 1-2weeks
COLO 205 細(xì)胞,人結(jié)腸癌細(xì)胞 5 × 105 1-2weeks
RKO細(xì)胞,人結(jié)腸腺癌細(xì)胞 5 × 105 1-2weeks
LoVo細(xì)胞,人結(jié)直腸腺癌細(xì)胞 5 × 105 1-2weeks
HT-29細(xì)胞, 人結(jié)直腸腺癌細(xì)胞 5 × 105 1-2weeks
Caco-2細(xì)胞,人結(jié)直腸腺癌細(xì)胞 5 × 105 1-2weeks
SW480細(xì)胞,人結(jié)直腸腺癌細(xì)胞 5 × 105 1-2weeks
SW620細(xì)胞,人結(jié)直腸腺癌細(xì)胞 5 × 105 1-2weeks
SW837細(xì)胞,人結(jié)直腸腺癌上皮細(xì)胞 5 × 105 1-2weeks
DLD-1細(xì)胞, 人結(jié)直腸腺癌上皮細(xì)胞 5 × 105 1-2weeks
KYSE30細(xì)胞,人食管鱗癌細(xì)胞 5 × 105 1-2weeks
KYSE-150 細(xì)胞,人食管鱗癌細(xì)胞 5 × 105 1-2weeks
KYSE-450細(xì)胞,人食管鱗癌細(xì)胞 5 × 105 1-2weeks
TE-1 細(xì)胞,人食管癌細(xì)胞 5 × 105 1-2weeks
ECA109細(xì)胞,人食管癌細(xì)胞 5 × 105 1-2weeks
EC9706細(xì)胞,人食管癌細(xì)胞 5 × 105 1-2weeks
MH97H細(xì)胞,人高轉(zhuǎn)移潛能肝癌細(xì)胞株 5 × 105 1-2weeks
HCCLM3細(xì)胞, 人高轉(zhuǎn)移肝癌細(xì)胞 5 × 105 1-2weeks
SMMC-7721細(xì)胞,人肝癌細(xì)胞 5 × 105 1-2weeks
BEL-7404 細(xì)胞,人肝癌細(xì)胞 5 × 105 1-2weeks
Hep G2細(xì)胞,人肝癌細(xì)胞 5 × 105 1-2weeks
Hep-3B細(xì)胞,人肝癌細(xì)胞 5 × 105 1-2weeks
BEL-7402 細(xì)胞,人肝癌細(xì)胞 5 × 105 1-2weeks
BEL-7404 細(xì)胞,人肝癌細(xì)胞 5 × 105 1-2weeks
SNU-739 細(xì)胞,人肝癌細(xì)胞 5 × 105 1-2weeks
HepG2細(xì)胞,人肝癌細(xì)胞 5 × 105 1-2weeks
97L細(xì)胞,人肝癌細(xì)胞 5 × 105 1-2weeks
HuH-7細(xì)胞, 人肝癌細(xì)胞 5 × 105 1-2weeks
QGY-7703細(xì)胞,人肝癌細(xì)胞 5 × 105 1-2weeks
HOC細(xì)胞, 肝卵圓細(xì)胞 5 × 105 1-2weeks
L-02(HL-7702) 細(xì)胞, 人正常肝細(xì)胞 5 × 105 1-2weeks
QSG-7701細(xì)胞, 人正常肝細(xì)胞 5 × 105 1-2weeks
GES-1細(xì)胞, 人胃上皮細(xì)胞 5 × 105 1-2weeks
MGC-803細(xì)胞,人胃癌細(xì)胞 5 × 105 1-2weeks
MKN-45細(xì)胞,人胃癌細(xì)胞 5 × 105 1-2weeks
AGS細(xì)胞, 人胃癌細(xì)胞 5 × 105 1-2weeks
AZ-521細(xì)胞,人胃癌細(xì)胞 5 × 105 1-2weeks
Hs 746T細(xì)胞,人胃癌細(xì)胞 5 × 105 1-2weeks
NUGC-3細(xì)胞,人胃癌細(xì)胞 5 × 105 1-2weeks
SNU-638細(xì)胞,人胃癌細(xì)胞 5 × 105 1-2weeks
NCI-N87細(xì)胞,人胃癌細(xì)胞 5 × 105 1-2weeks
HGC-27細(xì)胞,人胃癌細(xì)胞(未分化) 5 × 105 1-2weeks
BGC-823 細(xì)胞,人胃腺癌細(xì)胞 5 × 105 1-2weeks
SGC-7901 細(xì)胞,人胃腺癌細(xì)胞 5 × 105 1-2weeks
GBC-SD細(xì)胞,人膽囊癌細(xì)胞 5 × 105 1-2weeks
QBC939細(xì)胞, 人膽管癌細(xì)胞 5 × 105 1-2weeks
血液和淋巴系統(tǒng)Blood and lymphatic system
6TCEM細(xì)胞,人T細(xì)胞白血病細(xì)胞 5 × 105 1-2weeks
JURKAT細(xì)胞,人T淋巴細(xì)胞白血病細(xì)胞株 5 × 105 1-2weeks
CCRF-CEM細(xì)胞, 人急性淋巴細(xì)胞白血病T淋巴細(xì)胞 5 × 105 1-2weeks
MOLT-4細(xì)胞,人急性淋巴母細(xì)胞白血病細(xì)胞 5 × 105 1-2weeks
REH 細(xì)胞,急性淋巴細(xì)胞白血病 5 × 105 1-2weeks
supb15細(xì)胞,急淋白血病細(xì)胞系 5 × 105 1-2weeks
K-562細(xì)胞, 人慢性髓原白血病細(xì)胞 5 × 105 1-2weeks
K562細(xì)胞,人慢性髓性白血病細(xì)胞 5 × 105 1-2weeks
HL-60細(xì)胞, 白血病細(xì)胞 5 × 105 1-2weeks
TF-1細(xì)胞,人血液白血病細(xì)胞 5 × 105 1-2weeks
HSPC-1細(xì)胞,人造血干細(xì)胞 5 × 105 1-2weeks
Daudi細(xì)胞, 人Burkitt淋巴瘤細(xì)胞 5 × 105 1-2weeks
NAMALWA細(xì)胞, 人Burkitt淋巴瘤細(xì)胞 5 × 105 1-2weeks
Raji細(xì)胞 人Burkitt淋巴瘤細(xì)胞 5 × 105 1-2weeks
RAMOS細(xì)胞,人B淋巴細(xì)胞瘤細(xì)胞 5 × 105 1-2weeks
HUVEC[HUV-EC-C] 細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞 5 × 105 1-2weeks
MPCs細(xì)胞, 單核巨噬細(xì)胞 5 × 105 1-2weeks
THP-1細(xì)胞, 急性單核白血病細(xì)胞 5 × 105 1-2weeks
U937細(xì)胞, 人組織細(xì)胞淋巴瘤細(xì)胞 5 × 105 1-2weeks
HCAEC細(xì)胞,人冠狀動(dòng)脈內(nèi)皮細(xì)胞 5 × 105 1-2weeks
HemSc細(xì)胞,血管瘤干細(xì)胞 5 × 105 1-2weeks
人淋巴細(xì)胞 5 × 105 1-2weeks
循環(huán)系統(tǒng)circulatory system
HUV-EC細(xì)胞,人臍靜脈內(nèi)皮細(xì)胞 5 × 105 1-2weeks
眼、耳、鼻、喉和口腔系統(tǒng)Eye, ear, nose, throat and mouth system
CAL-27細(xì)胞,人舌鱗癌細(xì)胞 5 × 105 1-2weeks
Tca-8113細(xì)胞, 人舌鱗癌細(xì)胞 5 × 105 1-2weeks
HDPC細(xì)胞, 人牙髓細(xì)胞 5 × 105 1-2weeks
人牙周膜細(xì)胞 5 × 105 1-2weeks
PDLC細(xì)胞, 牙周膜成纖維細(xì)胞 5 × 105 1-2weeks
CNE-2Z細(xì)胞,人鼻咽癌細(xì)胞 5 × 105 1-2weeks
人耳周膜細(xì)胞 5 × 105 1-2weeks
SCC-9細(xì)胞, 人口腔癌細(xì)胞 5 × 105 1-2weeks
KB細(xì)胞,人口腔表皮樣癌細(xì)胞 5 × 105 1-2weeks
收到處理方式:
新鮮細(xì)胞到貨處理方式,客戶收到細(xì)胞后按以下方式操作:
取出25cm2培養(yǎng)瓶,75%酒精消毒,拆下封口膜,放入37℃,5% CO2細(xì)胞培養(yǎng)箱中靜置2-3h,以穩(wěn)定細(xì)胞狀態(tài)。
待細(xì)胞達(dá)到80%匯合時(shí)準(zhǔn)備進(jìn)行傳代培養(yǎng)。
細(xì)胞傳代,吸出25cm2培養(yǎng)瓶中的培養(yǎng)基,用PBS清洗細(xì)胞一次;添加0.25%胰蛋白酶消化液約1ml至培養(yǎng)瓶中,37℃溫浴3min左右;倒置顯微鏡下觀察,待細(xì)胞回縮變圓后吸棄消化液,再加入*培養(yǎng)液終止消化;用吸管輕輕吹打混勻,按1:2適當(dāng)?shù)谋壤M(jìn)行接種傳代(原代細(xì)胞必須以1:2方式傳代),然后補(bǔ)充新鮮的*培養(yǎng)基至5ml,放入37℃,5% CO2細(xì)胞培養(yǎng)箱中培養(yǎng);待細(xì)胞*貼壁后,培養(yǎng)觀察。之后每隔2-3天更換新鮮的*培養(yǎng)基。
凍存管到貨后處理方式,客戶收到細(xì)胞產(chǎn)品后,請(qǐng)按照下述方法操作:
細(xì)胞凍存管公司多贈(zèng)送一管,建議客戶先復(fù)蘇1支,如果復(fù)蘇效果不理想,先與技術(shù)人員溝 通后再?gòu)?fù)蘇另1支。
細(xì)胞收到后盡快復(fù)蘇,有效期為一周。
收到凍存管后如不馬上復(fù)蘇,可以暫時(shí)放置于-80℃冰箱,不建議放入液氮罐。
復(fù)蘇所用血清必須是胎牛血清。
復(fù)蘇請(qǐng)按照細(xì)胞復(fù)蘇 SOP 方式操作(細(xì)胞復(fù)蘇 SOP 另行發(fā)送)。
熱賣:DC2.4細(xì)胞,SW48細(xì)胞,RS4:11細(xì)胞,KGN細(xì)胞,A2780細(xì)胞,A2780/DDP細(xì)胞,HL-1細(xì)胞,COV434細(xì)胞等
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