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Invitrogen 28025-013 M-MLV Reverse Transcriptase 簡(jiǎn)介:
產(chǎn)品名稱(chēng):M-MLV Reverse Transcriptase
貨 號(hào):28025-013
規(guī) 格:40,000U
品 牌:Invitrogen
國(guó)產(chǎn)/進(jìn)口:進(jìn) 口
儲(chǔ)存溫度:-20°C
特惠價(jià)格:致 電
庫(kù)存狀態(tài):現(xiàn) 貨
銷(xiāo)售商:上?;鄯f生物科技有限公司
慧穎生物專(zhuān)業(yè)供應(yīng)Invitrogen 28025-013 M-MLV Reverse Transcriptase,常備現(xiàn)貨,*。主營(yíng)Life Invitrogen產(chǎn)品,囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規(guī)化學(xué)試劑、培養(yǎng)基及原料試劑、各類(lèi)胎牛血清、馬血清等,如有需要,詳詢(xún)。
Invitrogen 28025-013 M-MLV Reverse Transcriptase描述:
MMLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RT) is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, M-MLV RT lacks DNA endonuclease activity and has a lower RNase H activity.
Thermostability – Optimal activity at 37°C
Size of cDNA – MMLV can be used to synthesize first-strand cDNA up to 7 kb
Applications – Synthesis of first-strand cDNA (1), primer extension (2), sequencing dsDNA (3), cDNA libraries, and RT-PCR (4)
Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid (5).
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)12-18 as template?primer (6).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C (6).
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