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中文名稱:人軟骨糖蛋白39(HCgp-39)Elisa試劑盒
英文名稱:Human GP39 (Glycoprotein 39, Cartilage/Chitinase 3-Like 1) ELISA Kit
規(guī)格:48人份/96人份,定量/定性
保存溫度:4°C/-20°C
有效期:6個(gè)月/12個(gè)月
適合樣本:血清、血漿等其它生物樣品
人軟骨糖蛋白39(HCgp-39)Elisa試劑盒 Human GP39 (Glycoprotein 39, Cartilage/Chitinase 3-Like 1) ELISA Kit 相關(guān)產(chǎn)品》》
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人軟骨糖蛋白39(HCgp-39)Elisa試劑盒檢測(cè)原理》》
本試劑盒采用雙抗體夾心ELISA法。用抗人GP39抗體包被于酶標(biāo)板上,實(shí)驗(yàn)時(shí)標(biāo)本或標(biāo)準(zhǔn)品中的GP39會(huì)與包被抗體結(jié)合,游離的成分被洗去。依次加入生物素化的抗人GP39抗體和辣根過氧化物酶標(biāo)記的親和素??谷薌P39抗體與結(jié)合在包被抗體上的人GP39結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物,游離的成分被洗去。加入顯色底物,TMB在辣根過氧化物酶的催化下現(xiàn)藍(lán)色,加終止液后變黃。用酶標(biāo)儀在450nm波長處測(cè)OD值,GP39濃度與OD450值之間呈正比,通過繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中GP39的濃度。
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