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潮霉素B(Hygromycin B)Roche老包裝,大量庫(kù)存,*,訂購(gòu): 56980388 !
產(chǎn)品名稱(chēng):潮霉素B
英文名稱(chēng):hygromycin B
品牌:Roche 老包裝
貨號(hào):10843555001
規(guī)格:1G
市場(chǎng)價(jià)格:1933元
定義:潮霉素B由吸水鏈霉菌(Streptomyces hygroscopicus)產(chǎn)生的一種氨基糖苷類(lèi)抗生素,通過(guò)抑制蛋白質(zhì)合成能殺死細(xì)菌、真菌和高等真核細(xì)胞,用于篩選和保持轉(zhuǎn)化有潮霉素抗性基因的原核和真核細(xì)胞。
用途:
1)潮霉素B是氨基糖苷類(lèi)抗生素,它可以通過(guò)抑制蛋白質(zhì)合成殺死細(xì)菌、真菌和高級(jí)真核細(xì)胞。
2)潮霉素B通過(guò)干擾移位、促進(jìn)80S核糖體的誤譯來(lái)抑制蛋白質(zhì)合成。
3)哺乳動(dòng)物細(xì)胞選擇的有效濃度通常是50 μg/ml--1 mg/ml之間;對(duì)于植物細(xì)胞,其有效濃度是20–200 μg/ml;對(duì)于細(xì)菌,有效濃度是20–200μg/ml;對(duì)于真菌,有效濃度是200μg–1 mg/ml。
4)轉(zhuǎn)染中用的特定抗生素。
5)潮霉素B磷酸轉(zhuǎn)移酶滅活潮霉素B。
6)攜帶hph基因轉(zhuǎn)染的細(xì)胞對(duì)潮霉素有抗性?! ?/span>
7)轉(zhuǎn)染細(xì)胞穩(wěn)定或暫時(shí)表達(dá)該基因。
產(chǎn)品描述:
Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).
For life science research only.
Product Description
Formula: C20H37N3O13
LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
Molecular weight: Mr = 527.5
Working concentration: 50 - 1,000 µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.
Contents
Solution, 50 mg/ml, in PBS (phosphate-buffered saline), sterile-filtered
Quality
Purity: >80% (HPLC)
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